Sugar-Lectin Interactions: How Does Wheat-Germ Agglutinin Bind Sialoglycoconjugates?
نویسندگان
چکیده
منابع مشابه
Wheat Germ Agglutinin
Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...
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Partial characterization of normal human polymorphonuclear neutrophil (PMN) plasma membrane components which bind Concanavalin A (Con A) and wheat germ agglutinin (WGA) was accomplished. Plasma membrane preparations of PMNs, separated on 9% SDS-polyacrylamide (PAGE) gels, showed the presence of 24 protein bands after Coomassie blue staining. Lactoperoxidase-catalyzed cell surface radioiodinatio...
متن کاملImmunocytochemical localization of wheat germ agglutinin in wheat
Immunocytological techniques were developed to localize the plant lectin, wheat germ agglutinin (WGA), in the tissues and cells of wheat plants. In a previous study we demonstrated with a radioimmunoassay that the lectin is present in wheat embryos and adult plants both in the roots and at the base of the stem. We have now found, using rhodamine, peroxidase, and ferritin-labeled secondary antib...
متن کاملnocytochem ical Localization of Wheat Germ Agglutinin in Wheat
Immunocytological techniques were developed to localize the plant lectin, wheat germ agglutinin (WGA), in the tissues and cells of wheat plants . In a previous study we demonstrated with a radioimmunoassay that the lectin is present in wheat embryos and adult plants both in the roots and at the base of the stem . We have now found, using rhodamine, peroxidase, and ferritin-labeled secondary ant...
متن کاملDistribution of wheat germ agglutinin in young wheat plants.
A liquid phase, competition-binding radioimmunoassay for wheat germ agglutinin, with a detection limit of 10 nanograms, was developed in order to determine the distribution of this lectin in young wheat plants. Affinity columns for wheat germ agglutinin removed all antigenically detectable activity from crude extracts of wheat tissue; thus, the antigenic cross-reactivity detected by the assay p...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1980
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1980.tb04410.x